FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n02_MT_30976.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n02_MT_30976.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25547983
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTT205900.012.710704145
AGAGCGG155150.011.588271145
CTTACAT136500.011.4871311
CGGGAAC134450.011.0683661
GGGGAAT187950.010.3510151
GGGGATA172200.09.3505592
GGGGGAT147200.09.2219831
GATCTCG466700.09.196078145
CCGGGAA114200.09.1534941
GGGATAG147200.09.1233521
AGGGGAT158950.08.989252
CAGGGGA183100.08.9204071
CTCGGTG424550.08.811292145
TCGGTGG435500.08.706272145
ACATTCA235200.08.6893914
ATAGGGT882950.08.637705145
CGGGAAT109550.08.6143291
CGTGTAT108850.08.591719145
GAACGTA163250.08.5680454
ATCTCGG434500.08.55946145
Produced by FastQC (version 0.11.5)