FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n02_MT_30907.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n02_MT_30907.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26374456
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC282150.10697850981267631No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGAG175100.030.0476575
TTAGAGT208600.025.8474166
CTCTATT128150.023.478125145
AACGTAT634750.022.7771136
GGAACGT523400.022.320074
CCATTGT387750.022.212555145
GGTTAGA255800.022.1266024
CCGGGAA539950.021.2279911
TAGAGTG300750.021.0602867
GTATTCA690200.020.9577229
TGTACTC107000.020.0652852
TGTCCTA110400.019.8243065
CGGGAAC590700.019.757252
GAGTGGC278150.019.5667789
CATTGTA235400.019.556952145
CGTATTC746200.019.4528928
ACGTATT755450.019.396977
GAACGTA753100.019.3517195
GGGAACG632200.019.307243
GATCCTG225850.019.156425
Produced by FastQC (version 0.11.5)