FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n02_MT_30904.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n02_MT_30904.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28242557
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCT138550.019.6187673
TACTCTA150150.018.968794
CTCTAGT153650.018.7724026
CGTGTAT178300.018.053415145
ATATCGC167200.017.6412984
TATCGCA168400.017.1711795
TGCCGTA206650.017.008919
ACTCTAG185100.016.0526525
GTGATAT205050.015.7903181
CTAGTTG179100.015.7407038
TAGTTGT189250.014.8581919
TGTACTC189950.014.7779192
TCTAGTT211050.013.9415177
TTGTACT196000.013.8896411
GTAGATA231950.013.846407145
TCGCAGT209900.013.8107387
ATGCCGT265800.013.66006858
ATCGCAG239900.013.2920446
TGATATC253450.012.9642542
GATATCG255700.012.6430293
Produced by FastQC (version 0.11.5)