FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n02_MT_30900.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n02_MT_30900.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26624748
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTGTCCTACTCTCCCACAACCTGTCGGTTGCAGTACCATTGGCGCTG273860.10285918950293915No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCTA200300.028.871675
CTACTCT238950.024.2925939
CCTACTC250000.023.6826558
GTCCTAC269900.021.668096
TCCTACT275750.021.4711697
GTGTCCT276600.021.3266684
CGATCTA67500.020.6133354
GCGGTGT327700.018.7396131
ATAGCAC131150.017.2952185
CATAGCA148850.015.5307584
TGTACGG138400.015.191117145
GGTGTCC397250.015.0161483
ACGTCCG180700.015.005182145
CTATTAG119750.014.8265098
TAGCACC159450.014.5891686
CGGTGTC410000.014.1869982
AACGTAT261600.013.5463126
GGTCATA162850.013.5058381
GAAACGC289550.012.6391724
GGGGAAA414600.012.37818051
Produced by FastQC (version 0.11.5)