FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n02_MT_30889.TR5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n02_MT_30889.TR5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27069849
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAT150500.024.374945145
CGATCTA55350.020.1640174
AGTGTAT143950.016.519306145
CTATTAG119700.014.2278368
TCGTGTT182250.012.331723145
GGGGAAT223700.010.7087321
CGGGAAC276700.010.4677621
AACGTAT237300.010.2614585
AGAGCGG202500.010.0603145
TATTAGT179600.09.96689
GTAGATA251350.09.489572145
ATAGTGT873400.09.39643145
GTGTAGA717450.09.236005145
TCGATCT145300.09.179083
GAACGTA335100.09.1050354
GATCTAT153750.09.0972415
ACTACTC154550.09.0032267
GGAACGT283950.08.9856143
ATCTCGG478900.08.810646145
GGGGATA278200.08.7862882
Produced by FastQC (version 0.11.5)