FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n02_MT_30889.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n02_MT_30889.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25307483
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACATCTATGCGAGTGTTTGGGTGTCAAGCCCGAGCGCGTAATGAAAG1975600.7806386751301977No Hit
CGCAGGTGTCCTAAGGGGGACTCATGGAGAACAGAAATCTCCAGTAGAGC1026020.4054215901281056No Hit
CGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATACG893930.35322754143507673No Hit
GGAGTCTAACATCTATGCGAGTGTTTGGGTGTCAAGCCCGAGCGCGTAAT741440.29297263580103955No Hit
CTCAAACTTCCATCAACTTGAGTTGATAGTCTCTCTAAGAAGCCGGCGAC736400.29098112996855513No Hit
GTCTAACATCTATGCGAGTGTTTGGGTGTCAAGCCCGAGCGCGTAATGAA673620.2661742378726482No Hit
ACCAGGTCCAGATGAAATAAGGATTGACAGATTGAGAGCTCTTTCTTGAT648450.25622856291160995No Hit
CTCGAACAGGCATGCCCTTTGGAATACCAAAGGGCGCAATGTGCGTTCAA644490.25466380832894364No Hit
CCCGGATCGGCCCACGAAGGACCTTCAAACTAGAAGTTGGGCGGTAAAAC618190.24427162511578096No Hit
GTTTGACTCTCTTTCCAAAGTGCTTTTCATCTTTCGATCACTCTACTTGT575350.22734382553966348No Hit
GGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGACAAGAAACCA570920.2255933551353171No Hit
CCCCTATGTCTTTTCACAATGTCAAATTAGAGTCAAGCTCAACAGGGTCT527250.20833758932091348No Hit
CCGGAATACACGCTCATCAGACACCACAAAAGGTGTTGGTTCATCTAGAC525620.2076935110457251No Hit
CCAAATTTGACGATCGATTTGCACGTCAGAACCGCTGCGAGCCTCCACCA493850.19513991178024304No Hit
AAAGGATTCAATCCTGCGCACTGTACAATTGCAATACGCCGGCGAACAGC486380.1921882156356679No Hit
GTTAGTTTTACCCTACTGATGACCTTGCCCCAATGGTAATACCGCTTAGT480940.19003865378473236No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG477290.18859639261636568No Hit
AAGGAGTCTAACATCTATGCGAGTGTTTGGGTGTCAAGCCCGAGCGCGTA463360.18309209177380462No Hit
GCCGCTTACAGCCATTACGCCAGCATCCTAGCAGATGCGCGGACCTCAGT459940.18174071281604734No Hit
GCCGCAGGCTCCACGCCTGGTGGTGACCTTCCGTCAATTTCTTTAAGTTT444620.17568716730936854No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA440880.17420934353685036No Hit
GCTCGAACAGGCATGCCCTTTGGAATACCAAAGGGCGCAATGTGCGTTCA436360.1724233105283524No Hit
CTTGTCTGCTTAATTGCGATAACGAGCGAGACCTTACTCTGCTAAATAGC427470.16891051551827577No Hit
GCGAGTGTTTGGGTGTCAAGCCCGAGCGCGTAATGAAAGTGAACGGAGGT424130.1675907477642087No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGACAAGAA421900.1667095854613436No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCG415020.1639910219439839No Hit
CCGCAGGCTCCACGCCTGGTGGTGACCTTCCGTCAATTTCTTTAAGTTTC408640.16147002844968816No Hit
GCCGTTCTTAGTTCGTGGGGTGACTTGTCTGCTTAATTGCGATAACGAGC403720.1595259394227391No Hit
CCCCACTTCAGTCCTCAAAGTTCTCATTTGAGTATTTGCTACTACCACCA401810.15877122193463492No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT384130.15178514591909437No Hit
AGACAGGTTAGTTTTACCCTACTGATGACCTTGCCCCAATGGTAATACCG374560.14800365567765075No Hit
CCAGAACCCAAAAACTTTGATTTCTCGTAAGGTGCCGAGCGAGTCAGAAA371170.1466641309212773No Hit
CTCACCAGGTCCAGATGAAATAAGGATTGACAGATTGAGAGCTCTTTCTT366540.1448346325077053No Hit
CCCAAATTTGACGATCGATTTGCACGTCAGAACCGCTGCGAGCCTCCACC356700.1409464544538072No Hit
TTCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTA353360.13962668669974015No Hit
GCTGAACTTAAGCATATCAATAAGCGGAGGACAAGAAACCAACAGGGATT346590.13695158858745454No Hit
CCCGACTGTTCCTGTTAATCATTGCGGCGTCTCTAGAAACCAACAAAATA342640.13539078540524951No Hit
CAAAGATTAAGCCATGCATGTCTAAGTATAAGCAATTATACCGTGAAACT332410.13134850273336152No Hit
CTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATACGTAGTGTG330630.1306451534512539No Hit
GGAGAACAGAAATCTCCAGTAGAGCAAAAGGGCAAAAGTCCCCTTGATTT327570.12943602491010267No Hit
CAAGAAACCAACAGGGATTGCCCCAGTAACGGCGAGTGAAGCGGCAACAG326990.1292068436833485No Hit
GCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATGTACCTCCC311750.12318490938035996No Hit
GTTTTACCCTACTGATGACCTTGCCCCAATGGTAATACCGCTTAGTACGA311520.12309402716975054No Hit
CAGAAATTTGAATGAACCGTCGCCAGCGCAAGGCTATGCGATCCGTAAAG306270.12101954192757927No Hit
CTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGGAGAACTTTCC302690.11960494056244156No Hit
GGGGGACTCATGGAGAACAGAAATCTCCAGTAGAGCAAAAGGGCAAAAGT302370.11947849574768063No Hit
CGCGGACCTCAGTCCAGGCTGGTAATATGTCGTCTCCCCTATAAGGCCTC300150.11860128484527678No Hit
AGAAATTTGAATGAACCGTCGCCAGCGCAAGGCTATGCGATCCGTAAAGT299270.11825356160468428No Hit
TAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGACAAGAAAC298070.11777939354933084No Hit
CGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGCGAAGAGATTCG297380.11750674691750262No Hit
TCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAG291260.11508848983520013No Hit
GTGCTGTTTGACTCTCTTTCCAAAGTGCTTTTCATCTTTCGATCACTCTA290640.11484350300660084No Hit
CAGGTCCAGATGAAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATTT290240.11468544698814971No Hit
CTCGTTGAAAAGCAATAATTGCAATGCTTTATCCCCAGCACGACAGAGTT289350.11433377234709592No Hit
GTCCAGATGAAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATTTTTC285500.11281248316950365No Hit
CTAAGGGGGACTCATGGAGAACAGAAATCTCCAGTAGAGCAAAAGGGCAA284290.11233436371368895No Hit
CTGAACTTAAGCATATCAATAAGCGGAGGACAAGAAACCAACAGGGATTG282510.11163101443158135No Hit
CTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTACCGAATTCTGC282440.1116033546283524No Hit
CCGCTGCGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAAGCA281610.11127538839006629No Hit
CTCAAAGTTCTCATTTGAGTATTTGCTACTACCACCAAGATCTGCACTAG278280.10995957203646053No Hit
GGCCGTTCTTAGTTCGTGGGGTGACTTGTCTGCTTAATTGCGATAACGAG277620.10969877960601614No Hit
CTGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGGCACCTCTATGCT272430.10764800276661256No Hit
CTCTAATCATTCGCTTTACCTCATAAAACTGAATACGTTACTGCTATCCT267220.10558932312628641No Hit
GGGGAATCAGGACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCC265760.10501241865893973No Hit
GAACAATCCAACGCTTACCGAATTCTGCTTCGGTATGATAGGAAGAGCCG260670.10300115582414893No Hit
CAGGCATTGCCGCGAAGCTACCATCTGCTGGATTATGGCTGAACGCCTCT259430.10251118216695039No Hit
TGCTGTTTGACTCTCTTTCCAAAGTGCTTTTCATCTTTCGATCACTCTAC259410.10250327936602782No Hit
GCGCCATCCATTTTCAGGGCTAGTTCATTCGGCAGGTGAGTTGTTACACA253800.10028654370725056No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCTC166600.085.818162
TCTCTTG167900.085.120655
GATCTCT174000.082.3194053
CTCTTGG178800.079.850146
GGTAGGG92900.073.376181
CTTGGTT226300.063.2818838
ACGCTCG55100.061.3054163
CTAACAT546650.059.6932951
GACGCTC57700.058.4581032
TAACATC559050.058.2590332
AACGGAT57600.057.0927471
CGCAGGT307250.056.9653171
CATCTAT575550.056.3996855
CTCGAAC212200.056.2079051
GAGTCTA221050.055.5892032
ATCTATG587050.055.1588336
AGGAGTC138700.053.7630922
TCTTGGT272950.053.661187
GGAGTCT230150.053.2119671
AGTCTAA231500.052.88633
Produced by FastQC (version 0.11.5)