FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n02_MT_30849.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n02_MT_30849.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25599573
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG75900.027.128084145
TGTACGG89050.021.330935145
TCGTGTT161600.015.792244145
AGAGCGG151100.013.386969145
CGGGAAC90650.013.2917461
GCGTCGG104650.011.916034145
CGCGGTT64150.011.3147541
CGGGGAT74500.010.229981
GTTCCGG65350.09.9807445
TCTCGGG74950.09.189555145
GAACGTA115350.09.1100844
GGGAACG116300.08.9807732
TCCGGCA73650.08.9543287
GGTTCCG77800.08.9425964
CTCGGTG279200.08.802921145
CGTGTAT82850.08.750845145
GTGTAGA514950.08.686856145
CGTCCTG714900.08.6810255145
GCGATCC76900.08.6718623
TACGTCC782450.08.635785145
Produced by FastQC (version 0.11.5)