FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33009908
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4488861.3598523207032265No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA877520.2658353364692807No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTAC226350.019.4631399
TTCCCTA232850.018.9508868
CACTCTT235900.017.51312
CAGTCTA124200.014.85454525-29
TTTCCCT347700.013.62883957
ATCTATG90600.012.3161346
TCTATGC94050.011.9414957
TATGCGA88050.011.6855399
CTATGCG87650.011.6561648
CGGGAAC156150.011.5581351
TGTCCTA92500.011.0449367
GAACGTA188500.010.5704634
ATACGAG75800.010.3235535
GGAACGT187350.09.9403913
TACGGAC109900.09.8893615
CTTTCCC450300.09.8637136
ACACTCT433600.09.7681381
ACTCTTT453150.09.72267153
CCGGGAA178250.09.5943681
CGAGGGA106900.09.3538388
Produced by FastQC (version 0.11.5)