FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n01_neg.9.24.18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n01_neg.9.24.18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25791295
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCTA50800.028.8301124
GACATAC76100.027.2497679
GAGAATG377600.020.25593145
AACAGAC107350.020.1943725
AGACATA132950.019.5788638
AGTCTAA78150.019.3900325
TCTAACA80000.019.2140467
TAGCATA78200.018.3586128
GCTGAAT108900.017.709271
GTAGCAT85550.016.7810137
CTAACAT90250.016.6304728
GTCTAAC88150.016.5328776
GTTACGA88600.015.6295521
CTCCAAC165350.015.4780921
CTGGCTC122250.015.3614539
TAACATG92000.015.0531359
CAGACAT187050.014.5748187
TCCAACA156400.014.55561352
CAATTAG63950.014.3994069
TTACGAC97800.014.0848552
Produced by FastQC (version 0.11.5)