FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n01_neg.7.18.18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n01_neg.7.18.18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4232709
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT2473525.843822478700993TruSeq Adapter, Index 2 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA1116642.638121354432823TruSeq Adapter, Index 2 (97% over 37bp)
TCCAGCATTAGAGGTAACAACTAGAGTACAATTTTTTCCAATAGAATCAC144750.34197956911283056No Hit
GCCTTGTAAGCGATGTGATTCTATTGGAAAAAATTGTACTCTAGTTGTTA113310.26770089793557744No Hit
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA112720.2663069915744267TruSeq Adapter, Index 2 (97% over 35bp)
CCTTGTAAGCGATGTGATTCTATTGGAAAAAATTGTACTCTAGTTGTTAC82180.19415461823621705No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATGTCGTAT65400.15451097630382812TruSeq Adapter, Index 2 (97% over 37bp)
CCACCAACTACCTAATCTGCCATCGGCCGCTCCAATCGCGCGAGGCCCGA47930.11323717269483917No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATATCGTAT46310.10940983658456085TruSeq Adapter, Index 2 (97% over 37bp)
CGATGTGATTCTATTGGAAAAAATTGTACTCTAGTTGTTACCTCTAATGC45090.10652752173607966No Hit
GCGATGTGATTCTATTGGAAAAAATTGTACTCTAGTTGTTACCTCTAATG44930.10614951323136082No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG26000.075.846711
GCATTAG38000.071.55255
AGCATTA41750.065.299294
TAGAGGT50050.053.892879
CCAGCAT61100.046.276392
CAGCATT68150.043.7268263
AATCGGA47000.042.265772
TTAGAGG65700.041.7175648
GCCTTGT57200.038.0247541
CATTAGA72500.037.7042856
TAAGCGA60850.035.5093777
ATTAGAG77600.034.572237
CCTTGTA65450.032.899012
CTTGTAA66350.032.7832763
GTAAGCG69300.031.4934796
AAGCGAT71150.030.8787868
TTGTAAG77350.028.2152084
TCCAGCA98300.027.3628651
AACTACC36550.026.1862876
AGCGATG84250.026.0774579
Produced by FastQC (version 0.11.5)