FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n01_neg.11.17.18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n01_neg.11.17.18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27869132
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT532480.191064436452488TruSeq Adapter, Index 2 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA314030.11268022269226038TruSeq Adapter, Index 2 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATATC132800.040.181032
CATTATG432750.037.46011145
GATATCT152850.035.2434163
ATATCTT151300.033.4961174
CGTCTTG167200.032.60738145
TATCTTG159550.031.0372475
GTCTAGG78900.028.6697831
TGCCGTG198450.023.78287145
CGGATAT209650.022.3746511
TCTTGGC211650.021.7872717
TAGGTTC110200.020.5929684
GACACTC69800.018.3848112
ATCTTGG300050.018.0748336
ATTATCG188700.017.404465145
CTCAGAC209300.015.969031
GTAGTCA113650.015.9490698
GTTACGA136050.015.9341474
GATCCTG180500.015.7860925
GAGTCTA62000.015.5530694
CTCATTT280000.015.4320135145
Produced by FastQC (version 0.11.5)