FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n01_neg.11.16.18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n01_neg.11.16.18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24302773
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT365960.15058363915920212TruSeq Adapter, Index 2 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTATG274300.036.447834145
GGATATC113500.031.2999922
GATATCT124300.029.2812183
ATATCTT114650.028.8368174
TATCTTG120050.028.0231975
CACTATA130300.027.5444059
ATACGAC135250.025.8916191
ATAATAC93800.022.879443
CGTCTTG147500.022.118404145
CGGATAT163300.021.2666321
GTCTAGG61400.020.5461431
CTCACTA180450.020.411217
GACTCAC199150.019.768855
ACTCACT196850.019.5581676
ACGACTC197950.018.972773
TACGACT203550.018.379032
GAGCATT168000.018.2126488
CGACTCA223700.017.339814
GTTACGA95100.017.2299654
TAGGGAA201450.015.7996191
Produced by FastQC (version 0.11.5)