FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n01_MT_9424.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n01_MT_9424.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27590791
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATAG165450.010.2979291
CGGGAAC112750.09.7740771
TCGTATG390700.09.129645145
GAACGTA104700.08.8641374
GGGGAAT222400.08.8019271
ACCGCTC875750.08.717269145
CTCGTAT383150.08.439141145
CCGCTCA858800.08.382807145
TATCTCG460900.08.352569145
CGTATGC369550.08.141551145
GGAACGT116950.08.1216353
ACACGTA81650.08.080113145
CCGGAAT103600.08.0479791
CGGGTAT57200.07.98533771
CCGGGAT90600.07.9223821
GGGAACG119100.07.91362862
GGGGATA203550.07.8009123
TATGCCG326050.07.7824454145
CGCTCAT811350.07.7561054145
ATCTCGT455000.07.7438536145
Produced by FastQC (version 0.11.5)