FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n01_MT_7651.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n01_MT_7651.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24206958
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT251760.10400315479541047No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTG268850.018.957352145
ACATAAC141800.018.7658776
TAAGTTA123950.017.7229712
GCGCATG407400.017.706568145
CGTCTTG225400.017.46539145
ATGCCGG314400.017.110146145
AGTTAGA133200.016.4387654
CGTATGG166200.015.921874145
TCAGATT208200.015.9138742
GATTGAA209350.015.8967695
AGTCACA113200.015.81914145
GTTTGAT253450.015.7905111
CAGATTG214550.015.7817363
GAGTCTA79750.015.5455412
AACATAA185650.015.5438015
GATCATG147000.015.3395355
GCATCTG256400.015.240636145
CAACATA181750.015.2389624
AGATTGA270800.014.7526284
GTTAGAA169900.014.5522495
Produced by FastQC (version 0.11.5)