FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n01_MT_7651.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n01_MT_7651.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25696569
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGATG276850.019.195147145
TGTCCTA114950.011.3535325
TCTAGTT180450.011.0496877
GTACTCT145800.011.0397363
TACTCTA158350.010.7600614
GACTCAC105950.010.6755715
TGTACTC161700.09.9089152
TATGCCG800650.09.897133145
ACACGTA87000.09.749865145
AACCGAG144500.09.7342245
TAGTTGT192100.09.7003929
CGTATGC939750.09.597099145
GTATGCC853650.09.588399145
GTCACAC119050.09.500078145
ACTCACT127750.09.478366
GGGATAG133100.09.478111
CTAGTTG186050.09.3533058
GCCGTCT739000.09.025584145
CTCGTAT950950.08.988519145
CCTACTC166350.08.9789998
Produced by FastQC (version 0.11.5)