FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n01_MT_7431.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n01_MT_7431.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21955927
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA746810.340140500558232TruSeq Adapter, Index 2 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT654570.2981290655593818No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA295900.13476998716565236No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT283220.12899478122695524No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT239110.1089045340695476No Hit
CCTCCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTAC232780.10602148567901507No Hit
GGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGAT221830.1010342218754872No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT221390.10083382040758289No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG85450.038.6073954
ATACGAC109600.034.596851
TACGACT126900.030.6799162
ACGACTC128300.029.6124843
TAGTTGT150050.028.8969619
GTACTCT147900.028.7766423
CGACTCA139850.027.6853474
TGTACTC155450.027.4704672
ATAATAC132050.027.3439453
GACTCAC148700.027.3055675
CTAACCA184500.026.841728
GAGTCTA77750.026.7622742
CACTATA147750.026.7458259
TTGTACT157250.026.6031
GAGCATT145200.024.9683278
TACTCTA172250.024.8349884
GAAACTA209050.024.7292274
AACCGAG175200.024.2928335
GTCTAAC97150.024.2541481
TAGGGAA158950.024.0834581
Produced by FastQC (version 0.11.5)