FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n01_MT_7379.TR5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n01_MT_7379.TR5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23847309
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACATTCAGTAGCGAGCTTAACCGAATAGGGGAGGCGTAGGGAAACCG238780.10012869795917015No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCTA181050.039.6462255
GATCCTG120950.030.4525015
GTGTCCT253100.029.1906364
GTTAGCG76150.025.2302761
TGATCCT140000.025.169224
ACATTCA147200.024.6769474
AACGTAT458650.024.4876966
TTACATT149600.024.3766612
TTCAGTA151900.024.1528247
ATTCAGT163150.023.6429946
CCATTGT344000.023.18279145
GTATTCA492650.023.0331239
TACATTC154700.022.8243943
CGATAAT36250.022.6012234
GCTCAGA97350.022.4924185
CATAGCA60400.022.3273684
TATTCAC504000.021.8670439
ACGTATT523250.021.8384677
CTTACAT166050.021.8311771
TCCTGGC165250.021.5873247
Produced by FastQC (version 0.11.5)