FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n01_MT_6215.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n01_MT_6215.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24752195
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTG121400.022.8141965
TGCCGTG225100.022.287584145
GCGCATG302950.021.442205145
CTGGCTC199100.021.3045469
GCATCTG197400.021.044563145
ATTCTGG90950.020.0897716
CGTCTTG210150.019.526278145
GCCAGTA101800.017.7340034
TAACTTG93000.016.1377374
TGATCCT181500.016.0584894
CCTGGCT170450.015.2290818
GGGTTCA307150.014.8958929
AGTCACA135400.014.885333145
ATGCCGG367350.014.742562145
ACAGGCG142550.014.5979496
TCTCGTT387450.014.576532145
AACGTAT401200.014.4037166
CTAGAAC68350.014.3199281
GGGAAAT157400.014.2791561
TAGTCAT84900.014.26252659
Produced by FastQC (version 0.11.5)