FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n01_MT_6214.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n01_MT_6214.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21353197
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGG234730.10992733312955433No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCTA88000.028.0118082
AGTCTAA95000.025.5673333
TCCTCTA118400.023.5151529
TTACTTC115700.022.7476984
TACTTCC122550.022.1862135
GAGTGTC118050.022.04759145
GTCTAAC117300.020.8303264
TCTAACA111950.019.882895
TCAGACG66400.018.7812987
GTTACGA63700.018.6667464
GCCAGTA82350.018.6653654
TTTTACT152550.018.1549912
CCAATAG192300.017.6454948
CTTTTAC160750.017.4546031
ACTTCCT160750.017.4104396
CTTGTGT68950.017.3500061
CAATAGC197450.017.2954569
TTACGAC66250.017.1822095
ACACGGA117950.017.027314
GGAGTCT149850.016.8373011
Produced by FastQC (version 0.11.5)