FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n01_MT_30982.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n01_MT_30982.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22928319
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAACAAACATGCTAGTAAACTAGCGCCACTTGCGTTCAAAGATCTGA372930.16265038880521507No Hit
TGGATATCTAGGTTCCCATAACGATGAAGAACGCAGCGAAGTGCGATAAG281000.12255586639386865No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAGG136350.037.701846
TCTAGGT144950.034.9144257
AACATGC191600.031.8635189
GATATCT179900.031.8384723
TATCTAG169150.031.8046825
TGGATAT171250.031.1173521
CTAGGTT170850.029.1553298
TAGGTTC181000.028.2414959
GGATATC202000.027.8875162
CAAACAT242100.027.3428427
GAAACAA247850.026.8248123
AAACATG250850.026.2161778
AACAAAC248400.025.8899465
ACAAACA252600.025.661126
ATATCTA213950.025.1108974
GCCAGTA134500.024.2575264
AGTAGTC136700.024.0268787
CGTCTTG194850.023.105898145
GCGCATG347050.022.08079145
GCATCTG215650.021.448793145
Produced by FastQC (version 0.11.5)