FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n01_MT_30979.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n01_MT_30979.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25540686
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACATTCAGTAGCGAGCTTAACCGAATAGGGGAGGCGTAGGGAAACCG823350.32236800530729676No Hit
CTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCA309300.12110089760314191No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG256810.10054937443731934No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACAT213850.062.2785641
CATTCAG273800.049.8367275
ACATTCA278650.048.760764
ATTCAGT283150.048.2436836
TTACATT287000.047.0361982
TTCAGTA298850.045.199897
TACATTC326250.041.535433
TCAGTAG373550.036.1812448
CAGTAGC387250.035.0885439
AACGTAT586150.028.388165
CCGGGAA470200.025.8884941
CCATATT157450.025.8317972
GGTTAGA261100.025.7969744
GTATTCA646700.025.7651988
TATTCAC653250.025.6512019
CAATTAG102050.025.5785929
ACGTATT671850.025.0915076
GAACGTA697550.024.8937384
TATTCAG224900.024.7268775
TCAGATT152700.024.4988172
Produced by FastQC (version 0.11.5)