FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n01_MT_30978.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n01_MT_30978.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24080095
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT557910.2316892852789825No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA407690.16930581046295706No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA316240.1313283855400072No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT298180.1238284151287609TruSeq Adapter, Index 8 (97% over 36bp)
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT286930.11915650664999453No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCCG285650.029.519253
GCGCATG320350.026.931105145
TCCCGAG324800.026.4301225
TACGGAC253750.026.3444025
GGGATCC323400.026.072541
ATCCCGA329650.025.9311354
GGATCCC330250.025.3120042
CCCGAGG342700.025.1561836
CCGAGGC369800.024.6262327
ACGGACC278950.024.173176
GCTACGG278600.023.9683653
TGGGATC200200.023.9012132
ATGCCGG306050.023.33328145
AACGCCG243850.023.3105873
CTACGGA295850.022.7669544
TGCCGTG271150.022.753693145
CGTCTTG242800.022.57382145
CGGACCT315400.021.7702547
CGAACGC283800.021.4590511
ACGCCGG268450.020.9854564
Produced by FastQC (version 0.11.5)