FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n01_MT_30976.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n01_MT_30976.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25547983
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACAT137350.012.2991671
CGGGAAC134800.011.0796431
ACACGTA74700.09.4142145
ATTCAGT228200.09.3413366
GGGATAG137000.09.2085744
ATCGGGG379650.08.917966145
GGGGATA167150.08.8922413
CCGGGAA117650.08.8739921
GAATCGG550150.08.868828145
TCAGTAG175700.08.8311238
AGGGGAT157550.08.7893642
TCGGGGT236750.08.7886915145
AGAATCG698300.08.772976145
GAGAATG372500.08.758273145
ACTCCGG1264200.08.751269145
GGGAACG158600.08.6397492
AATCGGG495250.08.60766145
CGGAGAA1048000.08.578131145
CAGGGGA175350.08.5174551
CTCCGGA1172750.08.3827505145
Produced by FastQC (version 0.11.5)