FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n01_MT_30907.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n01_MT_30907.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26374456
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC265630.1007148735124622No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGAG170350.025.7500465
TGTCCTA103900.023.4470655
TTAGAGT193800.022.9714076
AACGTAT639900.021.4488125
CCATTGT399650.020.426332145
GGAACGT520650.019.8022444
GTATTCA698250.019.7402388
CGATCTA54000.019.7371394
GAACGTA759350.019.1631134
CTATTAG113650.018.8844138
CCGGGAA534650.018.7269671
TAGAGTG277350.018.7178617
TATTCAC741150.018.6270339
GGTTAGA254800.018.3251324
CGTATTC754750.018.1852327
GATCCTG231300.018.0555865
ACGTATT775900.018.0353496
CGGGAAC577050.017.8660891
CATTGTA242750.017.859682145
GAGTGGC265800.017.5403759
Produced by FastQC (version 0.11.5)