FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n01_MT_30904.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n01_MT_30904.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28242557
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTATG216100.020.43125145
TATCGCA167200.018.559855
ATATCGC172250.018.3523274
TGCCGTA207350.018.218619
GTGATAT203600.016.3092771
GTACTCT129550.014.9989133
TCGCAGT205900.014.8956247
TACTCTA139350.014.7245954
GATATCG238750.014.6071013
ATCGCAG245500.013.7925796
CGCAGTT223700.013.5484978
CTCTAGT152400.013.1787686
TGATATC258850.013.164082
TAGTTGT175300.012.2844629
CTAGTTG178300.011.95574958
TCTAGTT193350.011.512487
ACTCTAG178350.011.5048335
ACCATGC319050.011.0217355
TGTACTC180000.010.9959052
CATGCCG390650.010.8021417
Produced by FastQC (version 0.11.5)