FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n01_MT_30900.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n01_MT_30900.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26624748
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTGTCCTACTCTCCCACAACCTGTCGGTTGCAGTACCATTGGCGCTG278040.10442915741399691No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCTA193400.030.516595
CTACTCT229000.025.6155179
CCTACTC249700.023.8402848
GTCCTAC263800.022.8954776
GTGTCCT274750.021.8765284
TCCTACT271100.021.8244537
CGATCTA69250.020.4162674
GCGGTGT324750.018.5520631
GGTGTCC404100.015.0712863
CTATTAG126450.015.0234378
TGCCGTG192650.014.526121145
CGGTGTC416500.014.3606052
CGTATGG121700.013.939802145
TCTCGTT239050.013.647563145
ATAGCAC138800.013.424925
CATAGCA142400.012.98354
GCGCATG329800.012.684008145
AACGTAT254900.012.5159066
TATGCCG492850.012.28296145
TAGCACC161400.011.8149456
Produced by FastQC (version 0.11.5)