FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n01_MT_30889.TR5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n01_MT_30889.TR5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27069849
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA53200.017.8534344
CTATTAG114900.015.3342588
AACGTAT237300.010.38830955
CGGGAAC276050.010.242881
TATTAGT174000.010.1675879
GAGAATG391850.09.990939145
ACTACTC165850.09.8802137
CACTACT189200.09.3506556
GAATCGG641150.09.283589145
GGGGAAT211900.09.2037881
TCGATCT137350.09.132273
GAACGTA341300.08.8797774
ATCGGGG441800.08.812132145
GGAACGT285650.08.7821973
TGCGATA257000.08.7741419
GGGATAG125100.08.7511441
ATCTATT170550.08.3750576
GTTTGAT412000.08.3587561
TCCGGAG1267750.08.34933145
CTCCGGA1337300.08.337962145
Produced by FastQC (version 0.11.5)