FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n01_MT_30889.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n01_MT_30889.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25307483
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACATCTATGCGAGTGTTTGGGTGTCAAGCCCGAGCGCGTAATGAAAG2098920.8293673456186852No Hit
CGCAGGTGTCCTAAGGGGGACTCATGGAGAACAGAAATCTCCAGTAGAGC1083590.4281698025836864No Hit
CGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATACG863710.3412864092410929No Hit
CTCAAACTTCCATCAACTTGAGTTGATAGTCTCTCTAAGAAGCCGGCGAC848480.3352684263385656No Hit
CTCGAACAGGCATGCCCTTTGGAATACCAAAGGGCGCAATGTGCGTTCAA727880.2876145367755458No Hit
GTCTAACATCTATGCGAGTGTTTGGGTGTCAAGCCCGAGCGCGTAATGAA727870.2876105853750845No Hit
GGAGTCTAACATCTATGCGAGTGTTTGGGTGTCAAGCCCGAGCGCGTAAT713200.2818138808983888No Hit
ACCAGGTCCAGATGAAATAAGGATTGACAGATTGAGAGCTCTTTCTTGAT671540.2653523465767022No Hit
GTTTGACTCTCTTTCCAAAGTGCTTTTCATCTTTCGATCACTCTACTTGT623260.2462749851496492No Hit
CCGGAATACACGCTCATCAGACACCACAAAAGGTGTTGGTTCATCTAGAC598880.23664147082505202No Hit
AAAGGATTCAATCCTGCGCACTGTACAATTGCAATACGCCGGCGAACAGC557770.22039726352873576No Hit
CCAAATTTGACGATCGATTTGCACGTCAGAACCGCTGCGAGCCTCCACCA555020.21931062840188412No Hit
GGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGACAAGAAACCA550980.2177142626155276No Hit
CCCCTATGTCTTTTCACAATGTCAAATTAGAGTCAAGCTCAACAGGGTCT546020.2157543679867334No Hit
GTTAGTTTTACCCTACTGATGACCTTGCCCCAATGGTAATACCGCTTAGT519870.2054214557804899No Hit
GCCGCTTACAGCCATTACGCCAGCATCCTAGCAGATGCGCGGACCTCAGT505760.1998460297296258No Hit
GCCGCAGGCTCCACGCCTGGTGGTGACCTTCCGTCAATTTCTTTAAGTTT501920.19832869195249483No Hit
CCGCAGGCTCCACGCCTGGTGGTGACCTTCCGTCAATTTCTTTAAGTTTC500950.19794540610775083No Hit
AAGGAGTCTAACATCTATGCGAGTGTTTGGGTGTCAAGCCCGAGCGCGTA483480.19104230950189713No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG474800.18761249390150733No Hit
GCTCGAACAGGCATGCCCTTTGGAATACCAAAGGGCGCAATGTGCGTTCA465970.18412340729419832No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA457610.18082003650856943No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGACAAGAA455610.1800297564163137No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCG447560.17684887904498445No Hit
CTTGTCTGCTTAATTGCGATAACGAGCGAGACCTTACTCTGCTAAATAGC439770.1737707380856484No Hit
CCAGAACCCAAAAACTTTGATTTCTCGTAAGGTGCCGAGCGAGTCAGAAA424030.16755123375959594No Hit
CCCAAATTTGACGATCGATTTGCACGTCAGAACCGCTGCGAGCCTCCACC402600.1590833825710759No Hit
GCGAGTGTTTGGGTGTCAAGCCCGAGCGCGTAATGAAAGTGAACGGAGGT401140.15850647810372923No Hit
AGACAGGTTAGTTTTACCCTACTGATGACCTTGCCCCAATGGTAATACCG399570.1578861082313085No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT396280.15658609747954785No Hit
CCCGGATCGGCCCACGAAGGACCTTCAAACTAGAAGTTGGGCGGTAAAAC394970.15606846401912036No Hit
TTCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTA372040.14700790276140854No Hit
GCCGTTCTTAGTTCGTGGGGTGACTTGTCTGCTTAATTGCGATAACGAGC371900.14695258315495066No Hit
CCCGACTGTTCCTGTTAATCATTGCGGCGTCTCTAGAAACCAACAAAATA371630.14684589534249615No Hit
CAAGAAACCAACAGGGATTGCCCCAGTAACGGCGAGTGAAGCGGCAACAG366480.14481092410493765No Hit
CAAAGATTAAGCCATGCATGTCTAAGTATAAGCAATTATACCGTGAAACT361210.14272853606184385No Hit
CTCACCAGGTCCAGATGAAATAAGGATTGACAGATTGAGAGCTCTTTCTT361040.1426613622540021No Hit
GCTGAACTTAAGCATATCAATAAGCGGAGGACAAGAAACCAACAGGGATT357920.14142852531008318No Hit
CGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGCGAAGAGATTCG356540.14088323204642675No Hit
CCCCACTTCAGTCCTCAAAGTTCTCATTTGAGTATTTGCTACTACCACCA354300.13999811834310036No Hit
GGAGAACAGAAATCTCCAGTAGAGCAAAAGGGCAAAAGTCCCCTTGATTT339180.1340236008456471No Hit
CAGAAATTTGAATGAACCGTCGCCAGCGCAAGGCTATGCGATCCGTAAAG336180.13283818070726353No Hit
CTCGTTGAAAAGCAATAATTGCAATGCTTTATCCCCAGCACGACAGAGTT331260.13089409168031446No Hit
GCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATGTACCTCCC331030.13080320946970508No Hit
GTTTTACCCTACTGATGACCTTGCCCCAATGGTAATACCGCTTAGTACGA327180.12928192029211283No Hit
CTAAGGGGGACTCATGGAGAACAGAAATCTCCAGTAGAGCAAAAGGGCAA324160.12808859735280667No Hit
TAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGACAAGAAAC323840.12796215253804574No Hit
AGAAATTTGAATGAACCGTCGCCAGCGCAAGGCTATGCGATCCGTAAAGT318190.12572961127742335No Hit
CTGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGGCACCTCTATGCT314630.12432291271320818No Hit
CTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTACCGAATTCTGC313850.12401470347722846No Hit
CTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATACGTAGTGTG313680.12394752966938673No Hit
CTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGGAGAACTTTCC312220.12337062520204004No Hit
CTCTAATCATTCGCTTTACCTCATAAAACTGAATACGTTACTGCTATCCT307500.12150556418431654No Hit
CCGCTGCGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAAGCA301170.1190043276923272No Hit
GAACAATCCAACGCTTACCGAATTCTGCTTCGGTATGATAGGAAGAGCCG297020.11736449650089659No Hit
TCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAG296190.11703653026261047No Hit
GTGCTGTTTGACTCTCTTTCCAAAGTGCTTTTCATCTTTCGATCACTCTA293600.11601311754313931No Hit
CGCGGACCTCAGTCCAGGCTGGTAATATGTCGTCTCCCCTATAAGGCCTC292920.11574442231177239No Hit
GTCCAGATGAAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATTTTTC285640.11286780277596155No Hit
CAGGTCCAGATGAAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATTT283130.11187600126018064No Hit
GCGCCATCCATTTTCAGGGCTAGTTCATTCGGCAGGTGAGTTGTTACACA279690.1105167195015008No Hit
CTGAACTTAAGCATATCAATAAGCGGAGGACAAGAAACCAACAGGGATTG279610.11048510829781058No Hit
CTCAAAGTTCTCATTTGAGTATTTGCTACTACCACCAAGATCTGCACTAG276340.10919300034697248No Hit
GCGATGGCCAGAAAATGGTGTTGACGCAATGTGATTTCTGCCCAGTGCTC275720.10894801351837319No Hit
ATAAAACTGAATACGTTACTGCTATCCTGAGGGAAACTTCGGCAGGAACC274110.10831183804410735No Hit
CAGGCATTGCCGCGAAGCTACCATCTGCTGGATTATGGCTGAACGCCTCT272390.10763219716476743No Hit
TGCTGTTTGACTCTCTTTCCAAAGTGCTTTTCATCTTTCGATCACTCTAC269760.10659297884345116No Hit
CTATGCGAGTGTTTGGGTGTCAAGCCCGAGCGCGTAATGAAAGTGAACGG253970.10035371751509227No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCTC158600.087.264812
GATCTCT161950.085.5090263
TCTCTTG163300.084.4921655
GGTAGGG92850.081.909661
CTCTTGG177700.077.525056
ACGCTCG53750.067.310323
GTAGGGA115900.064.930732
GACGCTC56000.064.214042
CTTGGTT218300.063.273318
CTAACAT537050.061.1675221
AACGGAT53250.060.042741
TAACATC550900.059.6685032
CGCAGGT297850.058.4434851
CATCTAT565700.058.1495635
CTCGAAC220400.058.1254731
AGGAGTC139350.056.8135642
ATCTATG582400.056.433926
AAGGAGT143300.054.843431
CAGGTGT326250.053.1804163
CGAACTT34500.053.1693043
Produced by FastQC (version 0.11.5)