FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n01_MT_30849.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n01_MT_30849.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25599573
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAC87900.013.1145081
GAATCGG384950.09.944028145
GAACGTA113050.09.5560064
GTTCCGG66300.09.29548555
ATCGGGG253150.09.278972145
AATCGGG373900.09.055119145
TCCGGAG801350.08.911416145
CTCCGGA858300.08.902956145
GGGAACG114900.08.8968642
CGCGGTT71300.08.84653951
AGAATCG537200.08.812718145
ACTCCGG901850.08.690086145
CACTCCG961000.08.622945145
TCCGGCA76800.08.5911287
GGAACGT120300.08.5582193
CCGGAGA778100.08.488202145
CGGCACT89450.08.4294011
TTCCGGC80000.08.4287976
AACGTAT119750.08.294915
TCGGGGT153500.08.265372145
Produced by FastQC (version 0.11.5)