FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35000993
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG356033810.172105688544322No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC358670.10247423551668948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA96950.076.781296
CGGAGAT101300.075.4083569
TCGGAGA102150.073.431458
CTCGGAG133000.055.035547
TGGGCTC168200.037.807533
GGCTCGG187500.037.007765
GGGCTCG174100.036.30784
CGTGGGC154000.031.1402821
GTGGGCT214850.024.2550962
GCTCCGA36350.016.1672616
TACACTG111850.013.1733495
TATACTG137400.012.9952475
GTCCTAG52550.011.8426291
CTAGGAC55800.011.6183873
GCACGGA42300.011.4918596
TAGACAG154600.011.4086165
CTCCGAG52950.011.371457
GTATAGG75200.011.2938521
GTGTAAG91000.010.9420681
TGTACCG25250.010.9234145
Produced by FastQC (version 0.11.5)