FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n02_MG_9361.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n02_MG_9361.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29922254
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA245050.0111.277446
GGGCTCG232250.0105.39994
TCGGAGA265350.0105.066648
GGCTCGG246850.0104.9000855
CGGAGAT266300.0104.339599
CTCGGAG292600.093.941997
CGTGGGC221100.090.3938751
GTGGGCT336950.062.0870172
TGGGCTC431150.057.257043
GCCGACC105550.017.579447145
TACACTG221750.017.2283825
GTATAAG389550.016.0028415-19
TGGCTCG72250.015.2500274
CTACACT179400.015.1925354
TCCGAGA51250.014.8553538
TATACTG305250.014.8192595
CCGTATA61300.014.779952145
TACACCG50950.014.6551615
GCTCCGA50600.014.1854526
GTATTAG206650.014.1071471
Produced by FastQC (version 0.11.5)