FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n02_MG_7920.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n02_MG_7920.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24426167
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA149250.0110.3704456
TCGGAGA157150.0106.444278
CGGAGAT157500.0106.440319
GGGCTCG141600.0101.973444
GGCTCGG153300.0101.05655
CTCGGAG169250.097.119547
CGTGGGC132500.089.485251
GTGGGCT255450.048.212742
TGGGCTC371850.039.196443
CCGACGC24250.026.003248145
GCCGACC34900.023.8832145
CGGCTCG19800.019.7692224
TACACCG22250.018.5714915
GACGACA51100.017.446339145
TGGCTCG42950.016.708334
GATCGGA18650.015.9391026
TGCTCGG45250.015.860565
CCGTATA27950.015.559283145
TACACTG222750.015.1334235
CGACTAT21250.014.666606145
Produced by FastQC (version 0.11.5)