FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n02_MG_7651.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n02_MG_7651.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29605578
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA276500.0106.147476
GGGCTCG249900.0104.36524
GGCTCGG270450.0102.607595
CGGAGAT297650.0100.5615169
TCGGAGA308650.096.6699148
CTCGGAG308100.095.8292167
CGTGGGC242450.088.330011
GTGGGCT332850.067.0342942
TGGGCTC415850.062.8306353
TATACCG61600.016.0048925
GTATAAG435700.015.41939715-19
TATACTG297500.014.9127955
GAGATGT448500.014.67274810-14
TACACTG203350.014.3666485
CTATACT224550.014.3650714
TGGCTCG87150.014.1397714
CTACACT172100.014.1099264
AAGAGAC490450.013.84822120-24
GTGTAAG170550.013.394481
AGATGTG496950.013.3064310-14
Produced by FastQC (version 0.11.5)