FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n02_MG_7431.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n02_MG_7431.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26076730
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG262480.1006567924735962No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA272000.0100.4120566
CGGAGAT297950.094.350469
GGCTCGG274550.093.815315
GGGCTCG257750.093.50984
TCGGAGA307500.091.041918
CTCGGAG314750.087.2846767
CGTGGGC268550.075.82521
GTGGGCT312250.067.88232
TGGGCTC376250.064.746493
GTATAAG400550.015.79512615-19
GAGATGT404350.015.29962710-14
AAGAGAC445550.014.36195720-24
AGATGTG435450.014.09702410-14
TACACCG81400.014.071255
GATGTGT439550.013.89954810-14
GTATAGG121050.013.8983991
ATGTGTA454700.013.81276710-14
TATACTG258950.013.5216975
TACACTG171250.013.4615955
GTGTATA470600.013.42549515-19
Produced by FastQC (version 0.11.5)