FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n02_MG_7379.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n02_MG_7379.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36938266
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA271650.0104.062896
GGGCTCG257150.097.8132554
GGCTCGG275100.097.4201665
CGGAGAT296200.097.378819
TCGGAGA300350.095.741538
CTCGGAG337050.084.260947
CGTGGGC253250.082.957251
GTGGGCT386550.056.803312
TGGGCTC504900.050.4869163
CCGTATA83650.017.76314145
GCCGACC139900.015.180347145
TATACTG360550.014.8985075
GTATAAG446100.014.65656815-19
GTATAGA261550.014.445691
GTGTAGG165650.014.4032261
TACACTG272750.014.3522185
TACACCG68600.014.2659165
CTATACT245700.013.7478734
CTACACT207200.013.3987344
GTGTAAG211850.013.1791551
Produced by FastQC (version 0.11.5)