FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n02_MG_6852.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n02_MG_6852.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24594560
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG285670.11615170183975644No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA233800.0108.197266
GGGCTCG214250.0106.606794
GGCTCGG229250.0104.729975
CTCGGAG250250.0101.378847
TCGGAGA270450.095.5239948
CGGAGAT272650.095.4731759
CGTGGGC208850.090.4934541
GTGGGCT271150.073.0936662
TGGGCTC323800.070.86783
TATACTG251450.015.3085075
GAGATGT383750.014.650949510-14
GTATAAG400300.014.41753915-19
TATACCG72450.014.4083455
TACACTG167500.013.9790525
AAGAGAC414850.013.84141720-24
GTCCTAG70100.013.6563031
AGATGTG420550.013.44823610-14
GATGTGT425750.013.266952510-14
GTGTATA443000.013.05404415-19
CTATACT187450.013.031724
Produced by FastQC (version 0.11.5)