FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n02_MG_6214.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n02_MG_6214.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32538058
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA335450.0123.1313866
TCGGAGA351100.0119.9842768
CGGAGAT354950.0119.2365959
GGGCTCG310450.0117.9353264
GGCTCGG333700.0117.222245
CGTGGGC283000.0109.2148061
CTCGGAG383250.0108.063347
GTGGGCT439300.072.8204962
TGGGCTC583300.063.182743
CGGCTCG28300.036.371414
GCCGACC72250.034.309555145
CCGACGC132200.029.551815145
CGTCGGC22400.027.518541
TGGCTCG63150.026.5153184
GACGACA102400.025.977236145
GATCGGA25200.025.0304746
TGCTCGG61200.024.1642345
GCTCCGA35550.022.8417196
ATCGGAG37250.020.2432127
CCGTATA52100.019.755018145
Produced by FastQC (version 0.11.5)