FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n02_MG_3256.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n02_MG_3256.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27460447
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG282830.10299541008928223No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA227200.0103.201976
GGGCTCG210550.0100.354674
GGCTCGG230400.096.813275
CGGAGAT250600.096.350929
TCGGAGA251050.096.292348
CTCGGAG253550.094.025727
CGTGGGC211150.082.249951
GTGGGCT297250.061.427962
TGGGCTC368950.057.5558553
TATACTG274650.014.9919665
TACACCG66200.014.8926095
TACACTG199250.014.6257315
GTATAAG375650.014.46428215-19
CTACACT159850.014.238734
GTGTAAG170050.013.9015161
CTATACT199600.013.4731034
GTGTAGG135700.013.4661031
GAGATGT401100.013.41321510-14
GTATAGG119350.013.3666151
CCTACAC131700.013.3173873
Produced by FastQC (version 0.11.5)