FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n02_MG_30981.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n02_MG_30981.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29140546
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA165200.0108.796626
TCGGAGA176150.0104.221918
CGGAGAT176700.0103.571379
GGGCTCG158500.0101.207434
GGCTCGG171100.098.9287345
CTCGGAG191550.094.0996557
CGTGGGC157500.085.916431
GTGGGCT298400.047.1027952
TGGGCTC433950.037.6804123
CCGACGC61750.030.04975145
GCCGACC45150.022.3149145
GACGACA59700.020.518719145
CGGCTCG21550.017.8274334
TATACTG348600.015.0347595
TACACTG263050.014.7435175
TACACCG24350.013.9921495
CCTACAC130950.013.8926293
CTGCCGC135300.013.714502145
GCTCCGA31750.013.7011336
TCCGAGA43150.013.6107558
Produced by FastQC (version 0.11.5)