FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n02_MG_30978.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n02_MG_30978.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27225357
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA139150.097.953376
GGGCTCG131200.091.7152564
TCGGAGA153350.090.449358
GGCTCGG142450.089.973435
CGGAGAT155950.088.479219
CTCGGAG161950.085.151887
CGTGGGC137600.072.468811
GTGGGCT253950.041.332962
TGGGCTC353800.034.743593
GCCGACC55650.016.02071145
TATACTG286350.015.9238435
TATACCG29250.014.3746275
GTATAGG112900.013.8747971
GTATAGA202100.013.7076781
GTGTAGG127550.013.4751841
TACACTG231700.013.4222625
TCTATAC204750.013.16817953
CTATACT204550.012.9702674
CTACACT175350.012.8151384
GTGTTAG205900.012.7854821
Produced by FastQC (version 0.11.5)