FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n02_MG_30908.TR4_batch_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n02_MG_30908.TR4_batch_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20095480
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG259630.12919820775617205No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA92250.094.07556
TCGGAGA100800.087.8286748
CGGAGAT103250.086.379219
GGGCTCG93200.083.995314
GGCTCGG100050.082.814345
CTCGGAG115700.075.38777
CGTGGGC104000.062.824321
GTGGGCT188650.036.155882
TGGGCTC262900.030.4623743
CCGACGC79750.024.629923145
GACGACA55200.020.483774145
TATACTG242500.016.9790335
GCCGACC50550.016.919415145
CCGTATA27050.016.880983145
TACACTG175800.015.9163985
GTGTAGG95650.015.0870541
CTACACT125800.014.9233484
CTATACT151000.014.54497054
GTATAGA147500.014.2574661
GTGTAAG126100.014.0317251
Produced by FastQC (version 0.11.5)