FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n02_MG_30908.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n02_MG_30908.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26535560
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG306160.11537725226073994No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA312700.0110.8534246
GGGCTCG284350.0108.390464
CGGAGAT339650.0105.546049
GGCTCGG312750.0104.373655
TCGGAGA345650.0103.104588
CTCGGAG349200.0100.185747
CGTGGGC296250.086.754481
GTGGGCT355650.074.840062
TGGGCTC425850.072.962893
GTATAAG456250.017.63626715-19
GAGATGT470250.016.6431910-14
TATACTG262900.016.0479155
AAGAGAC519750.015.60913320-24
GATGTGT507500.015.38730710-14
AGATGTG513600.015.32031610-14
ATGTGTA543450.014.79102410-14
ATAAGAG530150.014.66564720-24
GTGTATA552000.014.60597315-19
TCCGAGA63850.014.5358378
TAAGAGA560000.014.50276320-24
Produced by FastQC (version 0.11.5)