FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n02_MG_30907.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n02_MG_30907.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25989448
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA128150.091.710086
CGGAGAT132700.089.607789
TCGGAGA135300.087.724048
GGGCTCG129300.080.841324
GGCTCGG138550.080.2111745
CTCGGAG154250.076.570457
CGTGGGC136850.063.5909581
GTGGGCT250550.036.4252852
TGGGCTC352550.030.5496483
GCCGACC60550.017.477438145
CGACGAA49700.015.750937145
TACACTG225300.014.6724585
TATACTG296250.014.6088225
GTATAGA184200.014.5276581
ACGCGTT19600.014.052921145
CTATACT191200.013.9516814
CTACACT169700.013.8825154
GTGTAGG119850.013.2514971
CCGTATA36650.013.250733145
TCTATAC185650.013.1565823
Produced by FastQC (version 0.11.5)