FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n02_MG_30893.TR5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n02_MG_30893.TR5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25297996
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA264200.0100.083066
CGGAGAT285550.094.765399
GGGCTCG252200.093.868834
TCGGAGA289650.093.0223548
GGCTCGG276200.090.548445
CTCGGAG296950.089.220087
CGTGGGC273700.072.917071
GTGGGCT290700.071.217072
TGGGCTC334150.070.904253
GTATAAG351400.017.1955315-19
GAGATGT372400.015.87997410-14
TATACCG61900.015.8098835
TATACTG197350.015.4275765
AAGAGAC405100.015.09438620-24
ATGTGTA414300.014.69398910-14
GTGTATA413300.014.69383315-19
GATGTGT404550.014.6609910-14
AGATGTG406050.014.5782610-14
CTATACT149450.014.4534214
ATAAGAG407650.014.42372720-24
Produced by FastQC (version 0.11.5)