FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n02_MG_30889.TR5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n02_MG_30889.TR5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31416988
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA255800.093.0796666
CGGAGAT270350.089.3112269
TCGGAGA278250.086.747668
GGCTCGG279600.080.5279545
GGGCTCG264350.080.258784
CTCGGAG320650.074.551767
CGTGGGC270350.064.162061
GTGGGCT336050.053.9494062
TGGGCTC410350.052.0865143
CCGTATA80350.015.966953145
GTATAAG353250.015.30315315-19
TACACCG105350.015.2756895
GTATAGG110200.014.6088111
TATACTG239300.014.4193825
GTATTAG192650.013.7017451
TACACTG194450.013.6071535
GAGATGT398350.013.60406410-14
GTATAGA182450.013.5138331
CTACACT155050.013.463874
TAGACAG283200.012.8496955
Produced by FastQC (version 0.11.5)