FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n02_MG_30785.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n02_MG_30785.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30369164
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA281850.0109.556476
GGGCTCG251750.0109.24044
GGCTCGG274500.0106.1894765
CGGAGAT305550.0103.4409269
TCGGAGA307850.0102.713338
CTCGGAG322350.096.854027
CGTGGGC251900.091.955311
GTGGGCT357600.067.340692
TGGGCTC444350.062.6341823
GCCGACC115450.017.39061145
GTATAAG448850.015.84009215-19
TATACTG315900.015.099625
CCGTATA73950.014.800205145
TGGCTCG82300.014.7967014
GAGATGT480000.014.63150510-14
TACACTG221450.014.5671345
AAGAGAC527400.013.90366320-24
GATCGGA34050.013.8402746
GCTCCGA59200.013.8390146
CTATACT212850.013.7923044
Produced by FastQC (version 0.11.5)