FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n02_MG_30783.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n02_MG_30783.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23638630
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA146100.0102.875646
TCGGAGA155600.098.6503458
CGGAGAT157450.097.399749
GGGCTCG141650.095.282384
CTCGGAG161150.094.217577
GGCTCGG152200.093.73395
CGTGGGC150150.075.737661
GTGGGCT250250.047.0128822
TGGGCTC355400.038.522173
TATACTG285800.016.0049155
GTGTAGG110900.014.9105791
TACACTG212650.013.9767025
GTATAGA178400.013.1310331
GTGTAAG161050.012.7893611
ATACTGT354550.012.7605856
CTATACT184550.012.7256654
CCTACAC113850.012.6682393
TACACCG26400.012.3564825
GTATAAG285950.012.2669215-19
GTATTAG175250.012.1255321
Produced by FastQC (version 0.11.5)