FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n02_MG_2397.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n02_MG_2397.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28881828
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG304610.10546770100562887No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA199450.0108.1098566
TCGGAGA210150.0104.6466758
CGGAGAT214000.0102.7324459
GGGCTCG190150.0101.057894
CTCGGAG223450.097.0532767
GGCTCGG210300.096.795015
CGTGGGC203400.079.797381
GTGGGCT323200.052.505972
TGGGCTC442900.044.1513753
GCTCCGA44150.014.4514136
GCCGACC82950.014.068125145
TACACTG242250.014.0347365
GTATAAG354200.014.00124115-19
TATACTG237000.013.3974135
GTGTAGG131150.013.2715741
CTTACAC179150.013.1889053
TACACCG40400.012.9195185
GTATTAG207250.012.8425421
GTAGGAC95450.012.7567873
CTACACT182750.012.4942454
Produced by FastQC (version 0.11.5)