FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n02_MG_2397.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n02_MG_2397.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22360055
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG225860.10101048499209864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA151900.098.611256
GGGCTCG143250.093.5589754
GGCTCGG159700.089.646945
CGGAGAT171550.089.1833049
CTCGGAG169600.088.922137
TCGGAGA174200.087.616928
CGTGGGC149300.074.795431
GTGGGCT222600.052.1023642
TGGGCTC295600.045.898343
TATACCG40400.015.7897255
TATACTG242050.015.5130765
GTGTAGG109350.013.7929991
GTATTAG173150.013.1917451
TACACTG173300.013.1760515
CTATACT173200.012.9316444
GTGTAAG138750.012.5427391
TACACCG49850.012.5056585
GTATAAG281100.012.44762715-19
CTACACT143350.012.3882964
CTTACAC134850.012.25404553
Produced by FastQC (version 0.11.5)