FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35000993
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG473160.1351847360444888No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA80350.063.1112986
GGGCTCG85550.061.6499444
GGCTCGG91500.057.4818655
CGGAGAT89850.055.2155729
TCGGAGA91450.054.2408078
CTCGGAG95750.053.387137
CGTGGGC103750.051.780421
GTGGGCT156350.034.29242
TGGGCTC207950.026.2355943
TATACTG209250.014.9425855
TACACTG166100.013.5832815
GTATAGA151200.012.0678841
CTATACT164900.011.9664834
GTGTAGA123150.011.6879941
CTACACT145000.011.5574564
TAGACAG232400.011.3626385
TACACAG217400.011.2122745
GTGTAGG85400.011.1512261
TAGACTG122450.011.0196585
TAGGACA121450.010.99103454
Produced by FastQC (version 0.11.5)